Unveil The Complexity Of The Lipidome In Metabolic Diseases
Identification and/or quantitation of lipid compounds in a biological sample is termed, Lipidomics. The number and diversity of lipid compounds in the lipidome is unknown, with new lipid compounds being discovered regularly. Mass spectrometry is uniquely suited for measurement and profiling the vast possibilities. Our laboratory is equipped with the state-of-the-art Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometry system and data analysis software, enabling us to offer targeted and untargeted lipidomics services capable of identification, quantitation, and structural elucidation of lipids. DNA Xperts has an experienced team who can support your quest in lipid purification and characterization.
1.Untargeted Lipidomics and Profiling
Through untargeted approaches we analyze hundreds to thousands of lipid species from biological samples to create a detailed lipid profile. After using general liquid-liquid methods for extraction of most lipids within a sample, they are separated chromatographically using HPLC or UPLC in order to simultaneously detect individual molecular species and identify changes in these lipids between samples. Detection and identification of lipid species is achieved using high-resolution mass spectrometry (HRMS) and tandem mass spectrometry (MS/MS).
Identification and relative quantitation of candidate lipid species is facilitated by the use of data-processing software and publicly available databases. Molecular identification is provided based on the LC-MS/MS data available. Data is reported as tables of mass-to-charge ratios (m/z), with ion intensity peak areas normalized to internal standards, and they can be visualized using powerful plotting and graphing tools. Statistical analysis can also be applied to sort out significant changes within complex lipid profiles.
Identify profile changes across all major lipid classes or within a single lipid class.
- Fatty Acyls
- Sterol Lipids
2.Targeted Lipidomics and Absolute Quantitation
Targeted lipidomics enables the focused analysis of a known collection of lipids. Precise method development allows for the inclusion of equal amounts of isotope-labeled or odd-chain internal standards selected from customizable lipid panels. Lipids from each sample are extracted using standardized protocols and analyzed by LC-MS/MS using analyte-specific collision-induced mass transitions from the molecular ion (or, in some cases, from a known adduct) to a characteristic fragment ion. Analyte concentrations are determined from calibration curves prepared from authentic standards, when available, or surrogate standards when necessary. This approach is especially useful for quantifying lipids present at low levels in samples, such as endogenous bioactive lipids (e.g., prostaglandins, leukotrienes, or endocannabinoids). Standard statistical analysis tools can reveal changing trends in lipid species among experimental groups, which can be visualized with a heat map or other available graphical tools.
TARGETED LIPID PANELS
Maximize data from precious samples with established targeted lipid panels offering the sensitivity, efficiency, and specificity needed to identify and quantify biologically relevant lipids.
- Eicosanoids and Oxylipins
- Eicosanoids derived from arachidonic acid
- Oxidized mediators derived from linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid
- Sphingoid bases, ceramides, monohexosylceramides, dihexosylceramides, and sphingomyelins
- Endocannabinoids and Endocannabinoid-like Lipids
- N-acylethanolamides, acylglycerols, N-acyltaurines, N-acylglycines, N-acylserines, N-acyldopamines
- Urinary Lipid Metabolites
- Indicative of the production, metabolism, and excretion of eicosanoids and other bioactive lipids
- Cholesterol Esters
- Cholesterol esterified with saturated or unsaturated acyl chains
- Short-Chain Fatty Acids
- Fatty acids with up to six carbons, many of which are produced by gut microbiota
- Specific acyl chains (e.g., 20:4) or oxidized acyl chains (e.g., 20:4-OH, 20:4-OOH)
Project Design/Advice Assistance
All of DNA Xperts’ Lipidomics services are performed by a group of specially trained and experienced scientists in a streamlined workflow. Please contact our technical support team with details of your project requirements at SUPPORT@dnaxperts.com and we would be happy to work with you on confirming the details of your lipidomics order.